2021年9月10-12日，菁因康生物科技將參加由中國蛋白藥物質(zhì)量聯(lián)盟、廣東省生物產(chǎn)業(yè)協(xié)會、廣州醫(yī)藥行業(yè)協(xié)會、振威國際會展集團(tuán)主辦的第6屆BTE廣州國際生物技術(shù)大會暨展覽會。誠摯邀請相關(guān)領(lǐng)域采購商及專業(yè)人士蒞臨展會現(xiàn)場，洽談合作、共贏未來。

杭州菁因康生物科技有限公司開發(fā)的糖工程畢赤酵母表達(dá)系統(tǒng)具有與高等真核表達(dá)系統(tǒng)（HEK293細(xì)胞）相同的諸多優(yōu)點(diǎn)，包括抗體空間折疊、人源糖基化修飾等；同時又具有生長快，易于基因操作等優(yōu)勢。

公司開發(fā)出IgG全長抗體酵母展示技術(shù)平臺，展示的IgG抗體在分子結(jié)構(gòu)、理化特性 和生物學(xué)功能方面與天然抗體相近。這項技術(shù)可以構(gòu)建大容量IgG全長抗體展示庫，通過FACS分選技術(shù)對抗體親和力及表達(dá)水平同時進(jìn)行篩選，選出的抗體可以直接進(jìn)行動物實驗和臨床試驗。在抗體成熟優(yōu)化應(yīng)用中，這項技術(shù)比噬菌體展示更具優(yōu)勢，噬菌體展示只能展示ScFv等抗體片段，通過結(jié)構(gòu)轉(zhuǎn)換形成的IgG抗體可能會失去活性。我們提供涵蓋抗體人源化、親和力成熟、表達(dá)生產(chǎn)在內(nèi)的抗體基因工程改造一站式服務(wù)。

Hangzhou Genekine Biotech Co. Ltd has developed proprietary platforms of glycoengineered pichia pastoris and full-length IgG pichia display system. There are many advantages of these two platforms. Similar to mammalian cell (HEK293) expression system, glycoengineered pichia pastoris contains eukaryotic internal quality control apparatus for protein correct folding and human-like N-glycosylation. Full-length IgG is displayed on pichia surface as close to native form in molecular structure, biophysical property, and biological function.

FACS sorting for IgG pichia display system is based on antibody affinity and display level. The isolated antibody can be directly expressed for animal study and clinical trial. Selection of IgG in pichia display has no need of format conversion. However selection of ScFv in phage display requires format conversion into full-length IgG, which may cause the lose of activity. Unlike the mammalian cell display, our platform can operate in large library size, easy genetic manipulation, fast generation time, and low cost screening. We will provide one-stop shop service for antibody humanization, affinity maturation, and antibody production.